Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTSSQLNSLRSLP-CFE----KVVDISLLIDGMSHTCIKVTTAL----QVFFVKKLNSET----AETEVLSSKYAAKHGLSPRIIYHDNAWLVTDYIVGITLDKNE-LSSSQCIDTGLTLMAKLHQLVPQPNAKTIPCLDTSESVRRLFTNPNSIPSQQ------KLFLADIT---ETLTLK------------IGIEQKRSDVTNIICHGDINYSNIIIDETRQSWLIDFECSHLAPVDFDLSMFIAINNI------------------SPLHIDEIVNRYMSLVPSY-----K----PNKILLTYYLLYSYFINGLWYLDNTNDAKINNHLRSLAIEQWSAFDSFVVKYHLDIPKLMPLIS 3MES Chain:A ((51-410)) ---TEIIIGICRKNIPGWKEINESYIEVKQIFSGLTNQLFVVSIVNELKHPRILFRIYGKHVKFYDSKVELDVFRYLSNINIAPNIIADFPEGRIEEFIDGEPLTTKQLQLTHICVEVAKNM-GSLHIINSKRADFPSRFDKEPILFKRIYLWREEAKIQVSKNNIDKELYSKILEEIDQLEELIMGGEKFSMERALELKLYSPAFSLVFAHNDLQENNLLQTQ-NNIRMIDYEYSAINFAGADIANYFCEYIYDYCSEKQPYFKFKYEDYPCEELRKLFISVYLSQTLQEQVMPSQQIVHIMTKAVEVFTLISHITWGLWSIA------VEFDFTEYANTRFTHYLQKKKELI-----------

General information: TITO was launched using: RESULT: Template: 3MES.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1087 -19302 -17.76 -68.69 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -17.76 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_3MES.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3mes-query.scw PDB file : Tito_Scwrl_3MES.pdb: