Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MIDTDSLKNARFYATKTEPITDVDIVAEAPLERPELIKGKDAPGILTFARVH-REGGSDLYQLVV-----------DKQGRDILGEEETARAAGKQLSNGYAVGSG----VLHKLTDPALPPVETVRAVG-EQSNTSWIYNDRVIVKYFRRLTPTPNPEIELLEELTAAGCTHSAPLRGWTSVD-----------------IEGHTYVTAMLQDLVPGTDGYDYVTGTPDAFDATALGEAIRDVHTVLASTCETGTLAPGALATNLDRRLTGLVGRAPQLKEYEEKIRALYSTLAETSVPTQRIHGDLHLGQTLVDGDSWALIDFEGEPAASLEERRRPDSPLRDVAGMIRSFGYAAAASGQDQS-----------WADARTAELIAGYGEA-------DEAILRAYIADKCAYEVVYELENRPHMVHVPLDALASLV- 4O7O Chain:A ((6-454)) TLATKLPWSDWLSRQRWYAGRNRELATVKPGVVVALRHN---------LDLVLVDVTYTDGATERYQVLVGWDKAAIGVADDRTGFDALYDVAGPQFLLSLIVSSAVCGTSTGEVTFTREPDVELPFAAQPRVCDAEQSNTSVIFDRRAILKVFRRVSSGINPDIELNRVLTRAGNPHVARLLGAYQFGDALAYALGMVTEYEANAAEGWAMATASVRDLFAEGDLYAHEVGGDFAGESYRLGEAVASVHATLADSLGTAQATFP--VDRMLARLSSTVAVVPELREYAPTIEQQFQKLAAEAITVQRVHGDLHLGQVLRTPESWLLIDFEGEPGQPLDERRAPDSPLRDVAGVLRSFEYAAYGPLVDQATDKQLAARAREWVERNRAAFCDGYAVASGIDPRDSALLLGAYELDKAVYETGYETRHRPGWLPIPLRSIARLTA

General information: TITO was launched using: RESULT: Template: 4O7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1680 36359 21.64 96.96 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 21.64 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_4O7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4o7o-query.scw PDB file : Tito_Scwrl_4O7O.pdb: