Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPSLDMITDESLRTAAAIDFVCQHFSLGELVGIGDRLKGSYNINLKITT-TNG-EFVVRFISSSVSEAHLQYVSKLLAKLENEGIP-VPLPVQDEKGMSFVELDGKRVQVTPFVQGRSF-LCREQQVHSSASMLRRFHHILGDEPPGPLPEW-SFYHKSSDLRERLNRLRELP----T---------IPSKEHAEVSRVMNRVLHKY------D-KDASLLPRTIIHGDWHFWNQKYIRNEVSCVLDFDFVRNGTRLFDIAYSIWVIYLLLPNYAS-TFDASFLDGYGKL---SDIEIYLLPAAVSRISLFFLCQSIHSADPAVKWKHQYRKQMPFLDWMEKEGKSRIRSLASAVQA 4PDY Chain:A ((21-307)) ---------------PLIPQAVVSKYDLA-IQQR---HAD---GNIEVWTDSKGRRYAAKRSSIAP--AHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRHGDFTYYVTEWVSGQPANFGLPEHVAQTAYTLAQFHEATRSFRTDWK---DDVFGLFQARW---RDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLA--YACFLHFDAVRNIPKSEYRAVEAILRFPYLPWR--------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1048 -40074 -38.24 -158.39 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -38.24 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: