Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KRCDVGIYNSDGTGPRAYKNIDIPASCSQWDFDFEYGGVTYTVRLHYEGCRTEIIGRPQLPSHLIIRG 2EM0 Chain:A ((1-46)) -----GSSGSSGMGEKTWK-------CRECDMCFS---QASSLRLHQNVHVGE---KPSGPS----SG

General information: TITO was launched using: RESULT: Template: 2EM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 76 -4305 -56.64 -93.59 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -56.64 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_2EM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2em0-query.scw PDB file : Tito_Scwrl_2EM0.pdb: