Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------KQIAQLKEYLRNPPC--KVTIYAPPLKQGQTQLRTVMGFASAGKNEDVPVKIASTGHTYQCRTDATCEEPVCHGLPNGWTYEGVY 2OPE Chain:A ((28-147)) FEKGYQSQLYTEMVGINNISKQFILKNPLDDNQTIKSKLERFVSGYKMNPKIAEKYNVSVHFVNKEKPRAYSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHL-ETLSSDVGCEAF-

General information: TITO was launched using: RESULT: Template: 2OPE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 234 14195 60.66 175.25 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 60.66 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_2OPE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ope-query.scw PDB file : Tito_Scwrl_2OPE.pdb: