Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERIKINSITIDEAKKHAVFVVKKELC-CNVNKVQYLGGGSFGYIYKVDIDRPPYTVVMKACRCD---NMCEREAFELNLLAKDSLIQIPKVYFTYLKDDIVSIDYICMEYVEGKDCFTDFSKLFLS-----KAKKRAFADKITSAMYAWHSKTNDKFGLVQNA-----N-YDEWLDYYKPFAFDILQKARNMVDRGELESYFLKVMELAWNNFDYIFSEKVEHASLIHGDLNVMNILADDKLNPIAIIDPLESKWADREYDLFQLKNLT--GNAFGLYEMYKSKYPTSKNCDLKVAFYAMFHEIYC-YIRSGRRT-K-INHLRCLLNLKIELNNVSIHS 5UXD Chain:A ((6-297)) -------------DLDALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGG-DWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST-------SELIAYPLLPGSPGL-TV-AADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDL-----------ARVGAEFEIAPALRERWEAWLAD---D-GCWPGHSVLTHGELYPAHTLVED-ERITAVLDWTTAAVGDPAKDLMFHQVSAPSAIFEVALQAYAEGGGRPWP-GLARHCTEMFSAAPLGYGLYALATGEAAHREAAAAALNPP--------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1234 -9054 -7.34 -33.28 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -7.34 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: