Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------NFCAYYIKTLEGWELVGWVKIGGQDELTWKGDSIGVKAEDTDCKVVLVNGQ------ 1GJW Chain:A ((573-636)) GKFENLTTKDLVMYSYEKNGQKIVIAANVGKEPKEITGGRVWNGKWSDEE-KVVLKPLEFALVVQ

General information: TITO was launched using: RESULT: Template: 1GJW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 124 10789 87.01 215.78 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 87.01 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.271

(partial model without unconserved sides chains): PDB file : Tito_1GJW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1gjw-query.scw PDB file : Tito_Scwrl_1GJW.pdb: