Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHPFDQLTPDLVLDAVES-LGYLSDARVLALNSYENRVYQVGIEDGEPLIAKFYRPDRWSDAAIREEHAFSAELAE-CEVPVVAPLSR-DGESLFAF----SGFRFALFPRRGGRAPEP--GNLDQLYRLGQLLGRLHAVGATRPFEH---REALAVDNFGHAS--LATLLEGNFIPRSLLPAYESVARDLLKRLDALFAEVPYQPIRLHGDCHPGNLLCRD----DVYHMVDLDDCRMGPALQDLWMMLAGER--HERLAQIAELVDGYNEFHDFDPRQLPLLEGLRSLRLMHYSAWLARRWDDPAFPPSFPWFGSERYWGEQVLVLREQLAALDEEPLRLF 6EF6 Chain:A ((27-349)) ---------DVTIAQQALTHYDVSDNASLRLLNLSENATYLVEDGE-HQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSG-PDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHTHSRE-SATK----AISYAAGSCALAERYLSSD--------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1471 -983 -0.67 -3.28 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -0.67 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: