Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIINEEVIWIVNSLVGEF--EIIKDHRNSSNRTGVLEINANNKRMFIKIHNR---LSRWSPEVYAYKNWTHILGEYAPRLIHSFND---DNFYGIITTPIYGKTVNEYQ---INDEDILEPIYYKAGELLKQLHNNFKGTYFGIPAIDGSPLESKAKTDPVDYINSALEDILKSGYDKGLFNNSDKELVKWCMKHSDVF--ANSKPVPTNWDFSQNNWMVDED-GKFTGFIDFENMLWGIDVDSFGIVIERYTPNRPKLRKALFEGYGLENSEEKQLQLKIVSVK--MAIADITYGASVGNDRIFSLGRNLMDNLKMPGFKIH 3N4U Chain:A ((5-299)) --TFDQVEKAIEQLYPDFTINTIEISGEGN-DCIAYEI---NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPK-QSQNQAAKDLARFLSELHSINISG-FKSN----------LVLDFREKINEDNKKIKKLLSRE-LKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIP-T-VLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIRSIK-------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1208 41258 34.15 147.88 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 34.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: