Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAERGRLLLTVVGELLAAEVCAAGTRIRGPDERARRERQRALRIRTALERLGPLYVKAGQVLSTR----EDLVPPAVARELESLHDRAAPCPFDRMSAVLEAELGPDWRRRFRHFDTERPLGCASIAQAYAAVLDDGRSVVVKVQRPGIAAQMTTDMRLLRGMTRTLARRTPVLSETIDFEAMLAVIFEAVRNELDLTLEAANMDEARAAVTAVPGIDVPGVVHATERVLIQRRAPGTSVRDADPAAFDDAERERVGTGLLTVMYQGYFIDHRFHADPHPGNVFVCPGGPTTLIDWGMIGRLDRHLSSTLLMTLLGLANNDAHAVARAWSEMGRPTPWA--DIGAFEQDMSVVVPRLA-------SIPLDRLRFGASLATLLRLSTRRGIRTNPMIGLLGKSFANMEGTVRHLAPHLSATDVLVRQTGRVLGEYAAQSLSQQQLGFTALQLLSGLETLLPHARAVGRSLSGGDLTLQHTAVTRPFSLVDHRSSARIRRAEHHLLGAAALVWLLRRRGRG 5YK1 Chain:A ((52-445)) --------------------------------VTAELMEKAANQLFTVLGELKGGAMKVGQALSVMEAAIPDEFGEPYREALTKLQKDAPPLPASKVHRVLDGQLGTKWRERFSSFND-TPVASASIGQVHKAIWSDGREVAVKIQYPGADEALRADLKTMQRMVGVLKQLSP----GADVQGVVDELVERTEMELDYRLEAANQRAFAKAYHDHPRFQVPHVVASAPKVVIQEWIEGVPMAEIIRHG-TTEQRDLIGTLLAELTFDAPRRLGLMHGDAHPGNFMLLPDGRMGIIDFGAVAPMPGGFPIELGMTIRLAREKNYDLLLPTMEKAGLIQRGRQVSVREIDEMLRQYVEPIQVEVFHYTRKWLQKMTVSQIDRSVAQIRTARQMDLPAKLAIPMRVIASVGAILCQLDAHVPIKALSEELIPGFA----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5YK1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1565 -6902 -4.41 -18.11 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -4.41 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_5YK1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5yk1-query.scw PDB file : Tito_Scwrl_5YK1.pdb: