Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRPPTPTADTVRRLVRTLLKDGAADASGPEVRPAAPGADPVTWLVGGRHVLRLARDRDAAARQRRELRLRDLVRTH-VPVAVPVSVAQGEWAPGLAYTLDTRVPGASGAEHD---V----SAVGEADLAGLLTGLREVPARQAETLGVPRAAPRSLEALRAAAEPAAERLAGADEFD---AARLRQLTRAAAVQLAAQSAPPVLVHHALTGGHLVVSADGRVRAVLGWAGTVLGDPAEDIAGLALAVG--SPAAVRAATLAGYGA----RPCLRGLWLARCDAVLQLAARLDGTGTAPLPRLRTRLRHAWEAILLERVTELRAEAGEDGERRA 3HAM Chain:A ((17-280)) ---------------------------KINELRYLSSGDDSDTFLCNEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQS-----DRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSD-ALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIFRN-NRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDRE------------------------------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1029 -27732 -26.95 -112.28 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -26.95 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: