Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRTEITFDFGTVPGAMRPYLEQA--TIYDSSCSEAAKTLFVEGA-EHAFLKICA------GGSLERECRMTRFMHGLGLA-PDVIAYESDGERDYLLTEAVEGQDGAEAVHL-EQPDRLAAVFGESLRMLHSLPVDGCPYP----HRTAEMLEEAIDK--------------GI----DPGLVHRLENFTSDDVLIHGDYCLPNVILDRFSFRSFIDVGNGGVGDRHFDLYWGLWTLRYNLKTDRYHERFLDAYGRQDVDADRLADFTSFIEHSA 1L8T Chain:A ((4-262)) -----------RISPELKKLIEKYRCVKDTE-GMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTE-KPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIK-PDWEKIKYYILLDEL--

General information: TITO was launched using: RESULT: Template: 1L8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1007 8401 8.34 37.17 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 8.34 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_1L8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1l8t-query.scw PDB file : Tito_Scwrl_1L8T.pdb: