Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLTEANVLDYLTGRGFAGPEFVVRGEWTVRNLSRRNRNFRVTRGGREFLVKQAGTWDLPGRASI-EREADLCRRAATDPCFGALRPLVPDVYS--------YDPDHSILIFEFLPDEASLSDVPERLDARTARLAGELMADYHRQMQSAALAEHFPGSLP---GYFSMHRWDSER----LVTRSQGQRELVRLV-KRHAAFAPALESAAAEWRPGALIHGDWKLENCLISND-GARMHVVDWELAGWGDARWDVATLLQSWWKRWVRDPAE-YRLEAMRPELRALLNPSEDEVAAILRFAAVRMLQSAWESLQDIPAIHGEAVRMAQVSLNILTNPEWAGEAVFGHD 4OCV Chain:A ((42-296)) -------------------------DSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQG------KETLDIVRTTSGDTWAEIDGGAWRVYKFIEH-TMSYNLV--PNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAA-GCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGF----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 927 -441 -0.48 -1.87 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -0.48 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: