Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKMKNHKITKLGGLNNSNYLLECEN---------NKYVLRIPSKDNK--NNFSEENFVLIFANLNKLSPPIIYHNKDNGILISKFLEDSKVNMSTFTSLEFLEKLSINLRKLHILKCEH--IF-NPFEHIRKNFHILKSKNF-N---FHQ-------DIDLVL-NKLNILEEKLSKNMTIGLCHNDLNSSNVLYYN-------KNVLFIDFEFSAMCDIFFDLATVSW-M-LD------------------EKKRYFLIKSYFGYYS--------------Y-ELMEKLENYLFVVKLWNASWSFLKSLNT---NSTYDYKLGGNMIIDDLLSTL 5FUT Chain:A ((32-375)) ------FHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----GAEAMVLESVMFAILAERSLGPKLYGIF--PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES---TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRK-

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 881 -28643 -32.51 -114.57 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -32.51 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: