Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------KCNISILKDGKYFTQKQEIAGWTVKF-----HDLDGNYVGKCKNAHTYACTYVTCEDLAPGYSPG 2EHF Chain:A ((1-73)) GSSGSSGEIEKGGCGDPGIPAYGKRTGSSFLHGDTLTFECPAAFELVGERVITCQQNNQWSGNKPSC----SGPSSG

General information: TITO was launched using: RESULT: Template: 2EHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 127 7752 61.04 138.42 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 61.04 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_2EHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ehf-query.scw PDB file : Tito_Scwrl_2EHF.pdb: