Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQVGEGEIELRNELPGGEVHAAWHLRYAGHDFFVKCDEREM-LRGFTAEADQLELLSRSKTVVVPKVWAVGS-DRDYSFLVMDYLSPRPLDA------------HNAFILGQQLARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAAEKGITFGNIDAIVEHVQQRLASHQPQPSLLHGDLWSANCALGP----DGPY-IFDP-ACYWGDRECDLAMLPLH-TDQPPQIYDGYQSVSPLPLDFLDRQPIYQLYTLLNRARLFG----GQHLATAQKAMDRLLAV 3TDW Chain:A ((5-297)) -KLHYTTMIMTQFPDISIQSVESLGEGFRNYAILV---NGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAISAGV--P---VTNLKNKILLLSEAVEDQ----VFPLLDE--SLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELFTRQVMAYRGEVDLDTH-IRKVSLFVTFDQVSYLLEGLRARDQDWISEGLELLEEDK--

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1214 -35640 -29.36 -132.99 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -29.36 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: