Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGAAEIIEAILGESGPDYESYSFEETPGDTRSDSHFVTVEYQGESYEVVVKLAPEV-D-STFAVEPFLHEYVAER--TDVPLPGILVFKEEPEQDVPPYFITERIHGDNLDE-HFESFSMDERGAIMFEIGEILGDMHSTIAFEGYGRLDLDDGRLIVRDLSWDWRAYFEELTQGHIDQLAETTFEDLQPTARERLADRLSVVPKQGTPRLVHDDFRPGNLLIEPDGPEISAVLDWEKTLAGDPLYNLAQIELLFIDSVFRDPDEREQLRERLYEGYGTETDFDADESYQACKPLYQFSTLVWRMAGFDSIYGDASPLARTRAEAYYREQFTNLARTLEPDR 5IQC Chain:A ((10-281)) --TNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN----N---EYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISD---ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL-DYTDISECTID-------NKQNVLEEY-ILLRETIYNDLT----DIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNN-RLTGIIDFGDSGIIDEYCDFIYLLEDSEEE------IGTNFGEDILRMYGNID----IEKAKEYQDIVEEYYPIETIVY-----------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1196 20670 17.28 77.41 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 17.28 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: