Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVKTKLFYEDIKPFFDIKSLQETKNGESHTVYILDN-----DYILKIYEEEN--LFNIDAEIELLNYTKK--LCVPKVIKDDIFIKGKRGLVFSKAKGESVVEF--VKSTHLEQIGQFLNSFHKMTKNKKHDN-----------LSAFG---KSQLKVMIEKTCNK--------------AFKDEFDCLK-----I--ELKNDGIIHGDLFLDNATFCG--DKLTCVFDFSDACEGDFIFDLAVVALSWCS---------NKEEINILLKAYDKEIKL---D----DFIIYLRYASLYYCVKRHLTNRDYDNIIFKNKFIDLI 2QG7 Chain:E ((111-427)) ------------------LEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIA-KKIYVFF---TN--GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSL-------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 798 14129 17.70 65.71 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain E : 0.60 3D Compatibility (PKB) : 17.70 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: