Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVSDGAVRQRLEAFCRNEFDASELTISRLERIPEGHSGFTYFVDFVDGPHARLVLRLPPPGARPQGPADVARQGRIMTALHHRGL--PVPEVHVISEDPGALDGRPFILMEAIDGVRADKAA------------AEADNAELARAAVAVMREIHAVDPADTGIGAEPARSLADELDRWSALIAKAPD---ELTKRAADLRALLSRSLPADHGPARLVHGDFTYGNLLFRG-GTVAAVLDWEIAQLGNPLLDLGSLCVIAKRHDYPNDPNPSGAIRATVEWLIDAYGAAGPVEWTVALSYYKYAAIVGYNTMLHRRGKRPDPIYDLMGDTVIGLIDGAIEILT 5UXA Chain:A ((6-255)) ---------KQVIEIAKKHNL--FLKEETIQFNESGLDFQAVFAQDNN--GIDWVLRLPRRED---VMPRTKVEKQALDLVNKYAISFQAPNWIIYT--------EELIAYKKLDGVPAG-TIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDDDM-WPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFG--------------EEGLEALILAYKEIG-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 902 -45873 -50.86 -197.73 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -50.86 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: