Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRTEITFDFGTVPGAMRPYLEQATIYDSSCSEAAKTLFVEGA-EHAFLKICA---GGSLERECRMTRFMHGLGLA-PDVIAYESDGERDYLLTEAVEGQDGAEAVHLEQPDRLAAVFGESLRMLHSLPVDGCPYPH----RTAEMLEEAID----------K-----GI-DPGLVHRLENFTSDDVLIHGDYCLPNVILDRF--SFRSFIDVGNGGVGDRHFDLYWGLWTLRYN----LKTDRYHERFLDAYGRQ---DVDADRLADFTSFIEHSA 4H05 Chain:B ((17-266)) ------------------------WVVVEDGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRLDVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFG-PECSAAFLREYGRGWDGAVSEEKLAFYRLLDEF--

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 928 -11699 -12.61 -54.16 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -12.61 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: