Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQQVISFLNENYPLH--IHTAKA----VTNEMYRC-IDDQGTYYARITNYK-P--YEEQLE-EVSWTNALYREGVGVAPAIPSCRGQIVELMPPKDIIVVVYKAAPGIHLPRGEWNADVLAELGRQIGRMHRITQSYEANHRLHHLGD---WHDQE--EYNFE-----QY---IPAEETAIRAIAANVLAEVKKLPRERATYGLIHGDLWLENILVDR--GSSLTMIDFQDCEKHYYMYDLAVPLYSALEF-SFAGA-GNIRDYGISIANALLEGYQE--EHTLDPEMLKQLPLFLKLKEIFEYNLMNMYWDRERLSEEQIRIMNLYRLRIEHHVQLLEWD 4OCV Chain:A ((24-322)) -TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDG-PRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEI--DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTME---------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1133 -19377 -17.10 -72.03 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -17.10 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: