Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQDEQVRAWLERELDGT-VVAFERQ--ERWRAAWFAELKNQGELRSYYVRGHRGGNFREMITLGQEADINRVLHR-HGVPVPRVHGMIDD---P----PAIVMDRLPGRVNLATALDDAEREAVLDQYVDAMCRMHAIDVNEFGALGLP--VPADNRAAALNMYATGERNYRRAKTAPSPMIEFMWRWLCRNVPAGRTQQGLIQADAA--QFLFEQ-GRMTGLIDFEAAYIGDPIAEFAAMRSRDCEEPLGDIGRIARRYEAVTGEPVD--RDAVEFHTAGWSLMTPFQWEDSVRNPAPGDSWLEYFTWLVGCGRWALEAIAAVAGAPLPTVEAPAQRPSSYSSAAYTNLMSLLDEWPTASAYEGFRADSARAVTRFAEHVHAFGADLEGAAVEEISELLGTRQPDLATAEAALEQFVLRASPDLDVALVQYFHRWTQRQQFLIAGTGARHEKLIHLSLQPLTADAWQAEGQSRRHA 5IGI Chain:A ((8-280)) --DTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDD---GRRWVLRIPRRAEV--SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV-VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREF----VVNDKRLHRWQRWLDDDSS-WPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALF--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1109 -7193 -6.49 -28.32 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -6.49 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: