Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAADPTVPYAGLSPERILEALES-VGLR------PDGRLLAL-NSYENRVYQVGMEE--------SAPLVAKFYRPGRWT-REAILEEHRFVQQLADAEIPAVPPLAFAAEFVGQLADDRGSCTLPEYGGFAFAVFPRCPGRVPAL---DQPDTLQWMGRFLGRIHALGTLEPYRHRLTLD-IDSFGHAPRDWLLAH----DMIPPELLPAWRSVVDQALEAVQACHARAGNVNLLRLHGDCHAGNVLWIDEADAPRYGAGALPGPHFVDFDDSCMGPAVQDLWMLLSGDR-----------------AAMTRQLGDILAGYEDFCSF-------------DPRELHLVEALRTLRLIHYSGWLARRWDDPAFKAAFPWFDTPRYWQDRILELREQVALMQEPPLVA 5FUT Chain:A ((9-376)) -------------RRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLG-PKLYGIF-------------------PQ--GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENL----RSLLES-TPSPVVFCHNDCQEGNILLLEGRE---NSEKQ--KLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS---IE--FGYMDYAQARFDAYFHQKRKL-------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1092 -28358 -25.97 -97.79 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -25.97 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: