Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSTRRPEIPADLRQWIAEMTGTDVVEVR-QVPGGASRQAWFVDAGTGARSRALFLRYDPREADP-GSAFHPLQVEAEIMAELHRHGVTVPQVIAAHPTLQAVLLERVGGDTWFRLIQDPDEQVRTARDFIGKLAALHRIDAQHLTIPSLGPAGPVAGHVRAEIAAMRARLSRYGKPAPLLAFCIDWLDRHVPDYDGPTVMVQGDTGPGNFMYSGGVVTAVVDWELAHFGDPMDDIAWLSLRTVQDTFTDFPARLAEYEQLSGHRIDD---DRVWYYRLFAE-TRLASISPGSIDTRASVPPASPDAGNSLIYGMLHRRLLVEALAHVVGIPEVDVELPPEGDADAPSEHSSVYEAAASAMSGAAARSTDA---LALRYVKGAARLVKY-LAEVDRIGAVVDAQETAELTAVLGWAPRSVSQGRAA-------------LAELAGRGDLTDRDYVTQLWRGIKHDDY-LTRTASGAL-ARRTWPPLRHPVPPPSVEQGDRDRWVSPSPKTTVS 4X8E Chain:A ((8-435)) PHRAELARQLIDARNRTLRLVDFDDAELRRQYDPLMSPLVW--DLAHIGQQEELWL---LRGGDPRRPGLLEPAVEQLYDAFVHPRASRVHLPLL-SPAQARRFCATVRSAVLDALDRLPEDA--DTFAFGMVVSHEHQHDETMLQALNLRSGEPLLGS---------------GTALP----------PGRPGVAGTSVLVPG--GPFVLGVDLADEPYALDNE-----RPAHVVDVPAFRIGRVPVT--NAEWRAFIDDGGYRQRRWWSDAGWAYRCEAGLTAPQFWNPDGTRTR---------------------------FGHVEDIPP-----------DEPVQHVTYFEAEAYAAWAGARLPTEIEWEKACAWDPATGRRRRYPWGDAAPTAALANLGGDALRPAPVGAYPAGASACGAEQMLGDVWEWTSSPLRPWPGFTPMIYQRYSQPFFEGAGSGDYRVLRGGSWAVAADILRPSFRNWDHPIR-----RQIFAGVRLAWDVD

General information: TITO was launched using: RESULT: Template: 4X8E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2058 326 0.16 0.81 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 0.16 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_4X8E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4x8e-query.scw PDB file : Tito_Scwrl_4X8E.pdb: