Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTATADIIAGLRAAG-LTGEGEVVLEPLTGGVSCDVWRVDGPAGPIVVKRPLEQLRVAA-EWHAPVERGASEVRWLRRARGVDPHLVPEVLAELP-GHGFAMRFLPDCPVWKDELIAGRVDPAFAAAVGRGIVAVHAATANSAADRD-------AFPTDAMFRA-LR---V-----DPFLLFVAQK---------DAELASALYALADDLSSRKVALVHGDVSPKNILVGADGPVFLDAECAVYGDPAFDLAFCTTHLLLKAVWLG-H--ERL----GEAAAALVEAYRAGIDW---------E-----DADALALRAGKLTAALLLARVEG---KSPAPYLTDPHHKQIVRDQAR----ALLLA---PTPIDALVANWKRTKE 2PUL Chain:A ((11-393)) --TLNESSAVALAVKLGL----TLTCQEIGDGNLNYVFHIY--DRALIIKQAVPYAKV--ESWPLTIDRARIESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAETLIHGDLHTGSIFASEHETKVIDPEFAFYGPIGFDVGQFIANLFLNALSRDGADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLDTIVPFDKRIGRKRLALETGTAFIEKRSEFKTITDVIELFKL---

General information: TITO was launched using: RESULT: Template: 2PUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1501 -1930 -1.29 -6.17 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -1.29 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_2PUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2pul-query.scw PDB file : Tito_Scwrl_2PUL.pdb: