Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNWSFGIDNDKLIGLVLEGKKTATSSLYNFDKIPVIGKESIIHFDNEKDACIVETVDYKIIKYNEMTEELAKLEGEGDLSLNYWKQVHLNFFKSVNPNFKEDDKIIFEIFKVTKNLVEERLKLGKSIASKNTDLLGNIVKVEEINSGFNNTLFNINDKYVIKVCTNKELENTFENEKD---FYLSNKDNCFIPKLYKYDDSKVDCDYIYEVIEKIKGKTLYYYWYKMNEADREKTIEKLIDIIKKFHSVNGKEYDWKNKIKGEIKNRIIKCKKYFKIKDYNMILESIDKYDEYLSDNKFALIHNDLHFDNVIYNNGKLTIIDFNDSIQAPIDFEFRLLYMCQEQPWKWADIEMDPYQKPEDYKNIWNYIKKYYKELNEIKFLEQRMIIYRIWNDTGHLKKYHNVELTKGIVENSKKLI 3GLF Chain:E ((1-334)) MRWYPWLRPDFEKLVASYQAGRGHHALLIQA--LPGMGDDALIYALSRYLLC---------------------QQPQGHKSCG-----HCRGCQLMQAG-THPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGA-------------KVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATRE---PERLLATLRSRCRLHYLAP-----PPEQYAVTWLSREVTMSQDALLAALRL--------------SAGSPG-------AALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQAPARLHWLATL-LMDALKRHHGAAQV-----TNVDVPGLVAELANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQ-------PGVVLPVPHL-

General information: TITO was launched using: RESULT: Template: 3GLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1533 -23245 -15.16 -70.23 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain E : 0.64 3D Compatibility (PKB) : -15.16 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_3GLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3glf-query.scw PDB file : Tito_Scwrl_3GLF.pdb: