Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQIVEDAGSLDVFRWPSSSPPSPENLEHAARLCQGALGSTSVAVVATPYNHAGWSAQDCIKVSFPSENRSFIAKVPRLVDENSATACKNEAQRASWASQHQIGPAVIAIDDLSGAFAMEF---LSGQTLSAQMML---------------QDHLKTCTIGLLRRIHDAPSQHYMQIYNAPAAVKRMLQAADKERLLDGDDGLLLESLIT---------WTFQKVGCQPTFQTLVPCHNDFHSQNIMLDQQGTLWAIDF---EDCDLGD----PMWDLGYLTANM-------GLEPLDLAGIYGCNMEQK----KRLEVFYYLAIGHC---AVWSAIHGPL-WTQHYRDCMLRLRRGWLRL 4EO3 Chain:A ((2-322)) --------ARVKHFELLTDEGKTFTHVD---------LYGKYTILFFFPKAGTSGSTREAVEFSRENFEKAQVVGISR----DSVEALKRFKEKN--------DLKVTLLSDPEGILHEFFNVLENGKTVRSTFLIDRWGFVRKEWRRVKVEGHVQEVKEALDRLIEEDLSL------NKHIEWRRARRALKKDRVPREELELLIKAAHLAPSCMNNQPWRFVVVDEEELLKKIHEA---LPGGNYWMKNAPALIAVHSKKDFDCALPDNRDYFLFDTGLAVGNLLVQATQMGLVAHPVAGYDPVKVKEILKIPEDHVLITLIAVGYLGDESELSEKHRELERSERVRKELSEIVRWNL--

General information: TITO was launched using: RESULT: Template: 4EO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1195 -35571 -29.77 -130.78 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -29.77 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_4EO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4eo3-query.scw PDB file : Tito_Scwrl_4EO3.pdb: