Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLCKWTVLNSEGEIIESGSCLNTEYMKHGD-IHVEFENDCTPIIFG-------- 1SV6 Chain:A ((1-261)) MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHINVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQPRIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNASCGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASLGEPLRTGDIILTGALGPMVAVNAGDRFEAHIEG------IGSVAATFSS

General information: TITO was launched using: RESULT: Template: 1SV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 61 2973 48.74 76.23 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 48.74 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.049

(partial model without unconserved sides chains): PDB file : Tito_1SV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1sv6-query.scw PDB file : Tito_Scwrl_1SV6.pdb: