Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------EQDCYYTVWTSNGNSW-----LRSIRDKTRSDHTLGIHGASVYFDEDCYPHYHSKE----------------- 1NWB Chain:A ((2-101)) QEQAQQFIFKVTDKAVEEIKKVAQENNIENPILRIRVVPGGCSGFQYAMGFDDTVEEGDHVFEYDGVKVVIDPFSMPYVNGAELDYVVDFMGGGFTIRNP

General information: TITO was launched using: RESULT: Template: 1NWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 80 6716 83.94 131.68 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 83.94 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_1NWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nwb-query.scw PDB file : Tito_Scwrl_1NWB.pdb: