Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGAAHS---ASEEVRELEGKTGFSSDQIEQLHRRFKQLSGD-QPTIRKENFNNVPDLELNPIRSKIVRAFFDNRNLRKGPSGLADEINFEDFLTIMSYFRPIDTTM-----DEEQVELSRKEKLRFLFHMYDSDSDGRITLEEYRNVVEEL-LSGNPHIEKESARSIADGAMMEAASVCMGQMEPDQVYEGITFEDFLKIWQGIDIETKMHVRFLNMETMALCH 2E30 Chain:A ((1-194)) MGSRASTLLRDEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPL----GDRIINA--FFPEGED---QVNFRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEKISRDELLQVLRMMVGV---NISDEQLGSIADRTIQEADQDGDS---------AISFTEFVKVLEKVDVEQKMSIRFL---------

General information: TITO was launched using: RESULT: Template: 2E30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 618 53328 86.29 289.83 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 86.29 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_2E30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2e30-query.scw PDB file : Tito_Scwrl_2E30.pdb: