Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAPFSIKQADIQQQLTAAIQKHFPAVEIADIRFELV-GGLSSKSWRIRGP--GIEWLARRQSPTERKMGVDRQREFALLRRMSAIGLAPRPLLWRDDWLIVEWVPGRIATSDEFLMMLANGEVARVLSQLHRLPRSGHP---------------LDIKMLFAQHWQLMDPHR----------------RSPVLLRAHHYFQRAPLPTPLALAPLHLDVHAENLLITP--QG-TMLIDWEYASDGDIAFELAFIVRAS---------QMDSMAQRYFLQSYQRHRRGFSVNRLQQQMRQWFPWVDYLVLMWFEIRWQQTKNPEFLADIPALYHRLQLGHRLQPD 2QG7 Chain:E ((109-453)) ----------------------------DSLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTD-EIINREREKKISCILYNKNIAKKIYVFFTNGRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASCLPI-----

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1028 -7636 -7.43 -29.26 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain E : 0.64 3D Compatibility (PKB) : -7.43 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: