Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLSSVAASFELGRDIEPSVVAMLQQAANNKSHGVYQSLSAEQLLPKLEAFIRAQGLAGAEVKGLRRMGGGASKEQFVFDLHSAAGPVQRCVLRMDPLESAVVTDRRKEYEVLNCMQS--VLPVPPVLWADIEGAELGRPALICGFIGGVTKPSGDSGNVSGLGALL--PERFRERLSAQFIDYLVAMHGVAVDF-KPEVFQVPDADPQQAARWQLNWWSSVWQDDAIE--G-SPLMGLAERWMRDNLPSARELVVVHADYRTGNYLFDEDSQEISTVLDWELAHIGDYHEDLAWIICHKGVTEDGQIMASGLMTPEELCERYRAATGRLVDAKTLHFYKVLSVYKCVAICLATAAQTASRKHSHQDVLLSWLTAAGHSFSAELLSLLKEVQ 5UXD Chain:A ((8-255)) ---------------------------------------------DALLDLAARHG-LDLDGGTLRTEEIGLDFRVAFARAHD----GGDWVLRLPRRPDVL-ERAAVEGRLLAMLAPHLDVAVPDWRIST-------SELIAYPLLPGSPGLTVAAD----G-EVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVR------SPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDE--RITAVLDWTTAAVGDPAKDLMFHQVSA--P---------SAIFEVALQAYAEGGGRP--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 984 -69760 -70.89 -290.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -70.89 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: