Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MVLASNVFV----SDPFNSANDPKMPFLAEALNPIKVQKYLTKALFNQFKNIQLHAIRVIRHKLGR--RCAIAYELTVDTQSLVIIGKARAKGLDHHSYELQRSLWQAGFSEDSSDGISVPEPLG-------------------IIPEWQMWLQKKVPGTIATDLLPGTEG--VAIASKIAEAAHKL-----HQTGIFTR-RCHTMSDELNILHERLPQ-VAQQYPQWEKRLEQILSKCDRLGANTPELSQCGIHR--DFYGDQVIVNGEHLYLIDLDLYCQGNP-----ALDIGNFIAHIT----------EYSLRILGNPNALADREAAMAERFIQFHGEAIRLGIQSYTTLTLVRHIYISTQISDRSSFTEAILELCEQRLR 5F86 Chain:A ((30-394)) NEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVLKRDLAPYK-STGVTRQMIESSARYG-TKYKIYGHRLYRDANCMF---------------PARCEGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFW-----AGGPATKLHPRGIGRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQGWKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEH--LRMKDIKCYWRKLLKRYVKLLQYEVKPEDQLIYIGP-------

General information: TITO was launched using: RESULT: Template: 5F86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1358 -15179 -11.18 -50.10 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -11.18 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_5F86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5f86-query.scw PDB file : Tito_Scwrl_5F86.pdb: