Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTSSQLNSLRSLPCFEKVVDISLLIDGMSHTCIKVTTALQVFFVKKLNSET------A-ETEVLSSKYAAKHGLS-PRIIYHDN----------AWLVTDYIVGITLDKNELSSSQCIDTGLTLMAKLHQLVPQPNAKTIPCLDTS--ESVRRLFTNPNSIPSQ----Q---KLFLADIT---ETLTLKIGIEQKRSDVTNIICHGDINYSNIIIDE--TRQSWLIDFECSHLAPVDFDLSMFIAINN--------------ISPLHIDEIVNRYMS--LVPSYKPNKILLTYYLLYSYFINGLWYLDNTNDAKINNHLRSLAIEQWSAFDSFVVKYHLDIPKLMPLIS 4OCV Chain:A ((24-331)) --TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAF-GDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDG-LRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADY---------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1063 -45318 -42.63 -174.30 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -42.63 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: