Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDTDSLKNARFYATKTEPITDVDIVAEAPLERPELIKGKDAPGILTFARVHREGGSDLYQLVVDKQGRDILGEEETARAAGKQLSNGYAVGSGVLHKLTDPALPPVETVRAVGEQSNTSWIYNDRVIVKYFRRLTPTPNPEIELLEELTAAGCTHSAPLRGWTSVDI--EGHTYVTAMLQDLVPGTDGYDYVTGTPDAFDATALGEAIRDVHTVLASTCETGTLAPGALATNLDRRLTGLVGRAPQLKEYEEKIRALYSTLAETSVPTQRIHGDLHLGQTLVDGDSWALIDFEGEPAASLEERRRPDSPLRDVAGMIRSFGYAAAASGQDQS----WADARTAELIAGYGEADEAILRAYIADKCAYEVVYELENRPHMVHV---PLDALASLV---- 4GEW Chain:A ((21-362)) ---EDSEVEIEILDEPEQGKLSMS--DEQKLHEFAIITATDEAFAQSILQ---DVDWDLKKALDVFYGSEAFAEARSAAVMGEDL-KGFEVSVMSWN---------------IDGLDGRSLLTRMKAVAHIVKNV----NPDILFLQEVVDRDLAPIDKLQSLYKIYYSNKGCQYYTAILVSKMFDVEKHDVIH-----FQNSGMY---RTLQILEGSI---GGLKVFLLNTHLESTREHRPQRCAQFGFCMDKVREIIAQ------------------------NPGALVFFGGDLNLRDEEVSRVPDGVKD----------AWEAAGSDNKTKFTWDTFKNDNK-QGFHGAKMRFDRLYWSGPLD-KVKFTLEGRQRIRSCLCFPSDHWAINATFFA

General information: TITO was launched using: RESULT: Template: 4GEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1356 -4940 -3.64 -15.88 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -3.64 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_4GEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gew-query.scw PDB file : Tito_Scwrl_4GEW.pdb: