Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCYGSMSCNPVEQRGEELKYMEKLIELYAQWAGE-------KPSTIEKLEGAGSNRVYYRFGNKS--------GQSVIAVIGTSRDENHAFIYLTEHFTDKQLPVPQILAVSDDELRYLQTDLGHTSLFDMLKSARDAGGRYTQKEKQLLTKTLQALSEMQVRGAVGLDFQQCYPQPEFNVESVLFDLNYFKYC-FLKATG-IDFH---ELMLEANFRMFAKDLT-AEPAECFLYRDFQARNVMLDND------GNPYFIDYQGGRKGPYYYDLASFLWQASAK----------------YPFKLRRDLVSAYYESLKQYTE-------VPSSRHFVERLNLFVLFRTLQVLGAYGFRGYFERKKHFIQSIPAAMQNLRELIKLGEHVFPYPYMMSVLKEMTELPQFANKEERKVKRTDGFKITDNNIFKANPVDGPATFSKYDDKGELIVRVNSFSYHKGIPEDPTGNGGGYVFDCRSTHNPGRYEPYKNLTGLDEPVIRFLEDDGEILVFLESVYQLADRHVARYIERGFTSLIFSFGCTGGQHRSVYCAQHLAEHIHDKFGIEVRINHREQKIEQTLEAK 5FTG Chain:A ((7-340)) ------------------RTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQ-CSLPDTTATLGDEPRKVLLRLYG--EAMVLESVMFAILAERSLG-PKLYGIFPQ--GRLEQFIPSRRLDTEE-----------LSLPDISAEIAEKMATFHGMKMPFNKE----PKWLFGT-MEKY-LKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 963 -6056 -6.29 -23.38 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -6.29 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: