Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------KVKKICHYSIQKLHGKT-----WYQVGTGQADAGKDTHIEGTKVFFSDQCLPQWNN-------------- 4RFU Chain:A ((7-123)) APIMTQGSLYNDSLSTNDFKSILLGSTPLDIAPDGAVFQLDRPLSIDYSLGTGDVDRAVYWHLKKFAGNAGTPAGWFRWG--IWDNFNKTFTDGVA-YYSDE---QPRQILLPVGTVCTRVDS

General information: TITO was launched using: RESULT: Template: 4RFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 89 -2019 -22.69 -44.87 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -22.69 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_4RFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4rfu-query.scw PDB file : Tito_Scwrl_4RFU.pdb: