Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEFDSEDFSTSEEDEDYVPSGGEYSEDDVNELVKEDEVDGEEQTQKTQGKKRKAQSIPARKRRQGGLSLEEEEEEDANSESEGSSSEEED-DAAEQE----KGIGSEDARKKKEDELWASFLNDVGPKSKVPPSTQVKKGEETEETSSSKLLVKAEELEKPKETEKVKITKVFDFAGEEVRVTKEVDATSKEAKSFFKQNEKEKPQANVPSALPSLPAGSGLKRSSGMSSLLGKIGAKKQKMSTLEKSKLDWESFKEEEGIGEELAIHNRGKEGYIERKAFLDRVDHRQFEIERDLRLSKMKP 6I27 Chain:A ((847-3803)) --EEDAGETETALDEEE----GAEEDVDMTNDEGKEDEENGPEEQAMSDEEELKQDAAMEENKEKGG----EQNTEGLDGVEEKADTEDIDQEAAVQQDSGSKGAGADATDTQEQD--------DVGGSG----TTQNTYEEDQEDVTKNN--------EESREEATAALKQLGDSMKEYHRRRQDI----KEAQTNGEEDENLEKNNERPDEFEHVEGANTETDTQALGS------ATQDQLQTID----------------EDMAIDDDREEQEVDQKELVEDADDEKMDIDEEEMLSDI--

General information: TITO was launched using: RESULT: Template: 6I27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 798 186178 233.31 772.52 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 233.31 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.020

(partial model without unconserved sides chains): PDB file : Tito_6I27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6i27-query.scw PDB file : Tito_Scwrl_6I27.pdb: