Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAVRALVASRLAAASAFTSLSPGGRTPSQRAALHLSVPRPAARVALVLSGCGVYDGTEIHEASAILVHLSRGGAEVQIFAPDVPQMHVIDHTKGQPSEGESRNVLTESARIARGKITDLANLSAANHDAAIFPGGFGAAKNLST-FAVDGKDCKVNKEVERVLKEFHQAGKPIGLCCIAPVLAAKVLRG---VEVTVGHEQEEGGKWPYAGTAEAIKALGAKHCVKEVVEAHVDQKNKVVTTPAFMCETALHYIHDGIGAMVRKVLELTGK 3L3B Chain:A ((3-211)) ------------------------------------------LNSAVILAGCGHMDGSEIREAVLVMLELDRHNVNFKCFAPNKNQKQVVDHKK-KESVGEVRNILVESARIARGSVYDIEQIRVEEFDMLVIPGGYGVAKNFSNLFDED--NDYILPEFKNAVREFYNAKKPIGAVCISPAVVVALLKDIAKVKVTIG-E--------------LIDKMGGVHVDCPTIKSVKDDVNRIFSCSAYMRNDSLYNVYLGIQDMI---------

General information: TITO was launched using: RESULT: Template: 3L3B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1219 -85777 -70.37 -431.04 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -70.37 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_3L3B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3l3b-query.scw PDB file : Tito_Scwrl_3L3B.pdb: