Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADSARYTFNMAGIIMHPGEKRVLLRAVQGGWMLPQVEWQADEYSSWKEVARFNREMQGLLDVCLSTVRCLGEDFERATMHFSRIYVMELHSSYDNLPADACWIDIEDLKEFMITPEEHLVWLEEWLAKRDEEERTPWYHPDWLAKAKVWIEQALAEPEAQPLVSIEQVGVAERGCLLRVQTKENAFYFKALPA------FFRHELTLLQHLAR-YRVGCVPALVAVDE------------REHRLLMHDFGSKHLDQS-LDLADWQEALRRYAILQIDHIQIDHIQIDHSAQVEQLEELGCPRLPLATLPDQLERLLTSEQSLLFDTPTALSLEQVRRLHDALPLIRSLCQELAGYGLPETLEHGDLHAQNIALSGG----DYLYFDWTDSCIAHPFFSLYPFLLDIEARWPEARIRLREAYLQPWERYAARAQLVEAFELAQRLAPLHLAMLYATLIIPHMKARWEMQHGVPLNLKVLLDRLHDAG 6EF6 Chain:A ((27-295)) --------------------------------------------------------------------------------------------------------------------------------------------------DVTIAQQALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSD-VTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLH----------EHSQRWTRPA-GFGRFSWDWEHCLGDTPR----WGRWQD--AEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD--PRLGEWQESWVAGYRSRREL-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1078 -25819 -23.95 -106.69 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -23.95 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: