Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSINFSNLSKNINFINFSKISRGNNKELKYIVKDSEMNRYLFKINSIIHLDNKVNEFRFAKRIEHLPFEKSEIVDLCVFNQNRYICSIYKWIDG-KILEDQIKNYPSNKQYSFGFKAGRSLKLIHDLPIRDEELKQVEELPVRKIIEYYHQNKRF----ENDNIIIDFILK----------NNSKIKDEKICFLHGHYSIKNFIIKNNDEISLIDYNQCYFGDYVQDF-SKNEIYNSYYSEQFAQGIIDGYFYNRKIPDTFWLKYAYYAAYNCITLFLWASKQEVISTKIK-IKEMIQNVLNQFNNFESIIPNWYKKNVLSKERQKFINSPYTLEKILGTPKKENKNDSKIKYFDEMYDDNDDDLLDEKMMDSFKEFLKNVAKK 3HAM Chain:A ((76-297)) -----------------------------------------------------------------------------VYQSDRF--NIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEI-DCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLK--YYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSELLQTQAEMF--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 728 23777 32.66 115.98 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 32.66 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: