Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence LKRVVICEVFIRMSGGLDLPIGKDFVPAGETTKIHAYTCETDTMCGAECDTPSNYRYSS 3N40 Chain:F ((339-388)) ---VTIREAEIEVEGNSQLQI--SFSTALASAEFRVQVCSTQVHCAAECHPPKDH----

General information: TITO was launched using: RESULT: Template: 3N40.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 104 -28170 -270.86 -563.39 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain F : 0.74 3D Compatibility (PKB) : -270.86 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_3N40.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n40-query.scw PDB file : Tito_Scwrl_3N40.pdb: