Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence AAVLPQLCKVEMFDKNARFVGMARGNWDKDFVIKGH-----TIKCNTRCETKDVVLP-KNWSVKG 1SS3 Chain:A ((1-50)) --------------DEAQFK-ECYDTCHKECSDKGNGFTFCEMKCDTDCSVKDVKEKLENYKPKN

General information: TITO was launched using: RESULT: Template: 1SS3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 78 90 1.15 2.05 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 1.15 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_1SS3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ss3-query.scw PDB file : Tito_Scwrl_1SS3.pdb: