Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSNNKLTRDELLSRFFPHYH-PVASLSQSGLSGGSVIISDGDRRHVLRQPHDPSAATYHFRRQYHALRRLPA---RLAPTPLFYS-----PCWLVVEYCAGEVKSE-----------LPASPQLSGLLYDLHQQPRFGWR-V-----SLTPLLAQYWQRCDPA-------RRKPRWLRWHQRLRRQGEPRPLRLAPLHMDVHAGNIIHNE----SGL-RLIDWEYAGDGDIALELAAVWITPG-ERRRLVEAYARRAAIDAQQLWRQVARWRPWVLLLMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG 3TDW Chain:A ((4-302)) -----NKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVN---GDWVFRFPKSQQG-ADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELFTRQVMAYRGEVD--LDTHIRKVSLFVTFDQVSYLLEGLR----ARDQDWISEGLELLEEDKANNFG

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1134 -24827 -21.89 -95.49 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -21.89 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: