Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHDKAFTFQTLRPDTIIDGLFD-LGMRVDSGLTPLNSYENRVYQFQD-EERHRYVVKFYRPERWSAEQILEEHQFALQLVEDEVP-VAAPLLF-NDSTLHQHQGFYFAVFPSLGGRQFEADNLDQMEWVGRYLGRLHQTGRKQRFTARPEIGVQEYLLE---PRQVFEQA-TLI-----PSGL-------KADFLKATDKLIAAVME------QWHGRGNTLRLHGDCHAGNILWR-D-GPLFVDLDDARTGPAIQDLWMLLNGDKA---EQRMQLETIVEAYEEFSPFNSDEIALIEPLRAMRLVYYLAWLLRRWDDPAFPVNFPWLTGEDYWRGQTSTFLEQVKVLQEPPLQLTPMY 4PDY Chain:A ((22-301)) --------------LIPQAVVSKYDLAIQ-Q---RH--ADGNIEVWTDSKGRRYAAKRSSIA---PAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRHGDFTYYVTEWVSGQPANFGLPEHVAQTAYTLAQFHEATRSFRTDWK-----DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLAYACFLHFDAVRNIPKSEYRAVEAILRF--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 978 4310 4.41 17.66 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 4.41 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: