Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRRHMNRFTIEQVLDIAEVIHSRGSRKVWRIG-DYILKQTPYE----GVNNEVATLRFVQQH--TTIPVPVVYDEWLSPDRRFHYILESRIPGKSLKQ-CWGVLSRKGRERIARTVLKYMQELSQFRSDRLESISSNKLRLNNFVPKPYKVLLNIWRTDNDIFDNEYRPALLS-SGVDDGVILVLKNTMPPCQGQYTLTHGDLFTGNIIVDPVKEQVTGIIDWESAGFWPAWFQYARITLGAGGWDDEWKALLSRAMKATHLPPHSKHGRIWWDAVYELLDHPGSSLAREWIKEIIRYLAGENVSLNNYDGFGL 5IQI Chain:C ((28-257)) --------------VDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDE--LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ-------------NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF-EGKKCLCHNDFSCNHLLLDGNN-RLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIG--TNFGEDIL-RMYGN---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 874 -29816 -34.11 -134.91 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -34.11 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: