Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTRRPEIPADLRQWIAEMTG-TDVVEVRQVPGGASRQAWFVDAGTGARSRALFLRYDPREADPGSAFHPLQVEAEIMAELHR-HGVTVPQVIAAHPTLQAVLLERVGGDTWFRLIQD-------PDEQVRTARDFIGKLAALHRIDAQHLTIPSLGPAGPVAGHVRAEIAAMRARLS-RYG---KPAPLLAFCIDWLDRHVPDYDGPTVMVQGDTGPGNFMYSGGVVTAVVDWELAHFGDPMDDIAWLSLRTVQDTFTDFPARLAEYEQLSGHRIDDDRVWYYRLFAETRLASISPGSIDTRASVPPASPDAGNSLIYGMLHRRLLVEALAHVVGIPEVDVELPPEGDADAPSEHSSVYEAAASAMSGAAARSTDALALRYVKGAARLVKYLAEVDRIGAVVDAQETAELTAVLGWAPRSVSQGRAALAELAGRGDLTDRDYVTQLWRGIKHDDYLTRTASGALARRTWPPLRHPVPPPSVEQGDRDRWVSPSPKTTVS 5UXD Chain:A ((4-256)) -----PEDLDALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHD---GGDWVLRLPRRPDV----LERAAVEGRLLAMLAPHLDVAVPDWRISTS--ELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEV-----RSPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCW-PGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA---PSAIFEVALQAYAEGGGRPW--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 981 -18438 -18.79 -76.82 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -18.79 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.185

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: