Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEKDRAVVTELLQKAGREDFIPLQENQLAGDGSSRRFYRLASGG---------ETLIAVLPAGEEAKDRAESRAAYHIGGYLLACGTPVPAIYGWDEASGGLLFQDLGNTRLQDVAHTEEGRQLYRQLVRALAGMNCRARGIDSSWCWDSPCYDRSLMLERESGYFLRAFWQG---L--LGQEVPIGLAEEFSSLADAVS-LYSTDFFLHRDFQSRNIMIHA-------GRPSIIDFQGGRRGPLGYDLASLLYDPYVD----------------LDEDFRALLWEDYLAALGAQI--------DISREDFAKQYSLLACQRSLQIIGAFSWLSAVAKKTFFVQFIRPSLLNLEKILSAPYYQNYRVLRQTVRRGLSLYR 5FTG Chain:A ((30-344)) ---------------------DEFHISVIRGGLSN-MLFQCSLPDTTATLGDEPRKVLLRLYG-------EAMVLESVMFAILAERSLG-PKLYGIFPQ--GRLEQFIPSRRLDTEELS--LPDISAEIAEKMATFHGMKMPFNKEPK--WLFG--TME--KYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLW---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 837 7934 9.48 32.51 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.48 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: