Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMLLVLFDKQGQILLCRNPSGYSVPTNGAEVDEGFGFGSPQEENRWFWENCGLLVYRRYICFYKDYCAAVFELYGEGNVGKEFIWMDSGSLDKFIVGNSLTVLLKAVMKNYNCSKTVSWVNKDGFQPYLNWALKAAGSDTQLTYGKIEQIKNTYVSTIFRFQAGE---------EIFYLKIPGKTY---LNDAVHIEKFLVNSTYGLPEITAVYPDGNAFLAKAMGGHDLSV-E-TDMVFLEQVVEQWADMQQRFTEKQDAFSSKIPLLHNYTAEGIMKRIDNFPSEVSDIFAFLNEQSNAVDAAKTGQMIREVKRLLSLLAKCKVPDTLCHGDLRPGNIRVVNG-------FCFLYDWGMGMYGHPFYDVVHFLHVTRRQLS------------QEMQEKIKNTYLKQWEMYGTG--------------KDILRNYETVERLKDFFMVLADADWLMETIEAVGGKVPEGSMDSWLLMRRVYYFRRVLGRFLAGDNN 5FTG Chain:A ((33-343)) --------------------------------------------------------------------------------------------------------------------------------------------------HISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---EAMVLESVMFAILAERS-LG-PKLYGIFP--QGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEP-----K-WLFG--TMEKYLKEVLRIKFTEE-SRIKKLHKLLSYNLPL---ELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGL-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 819 -15805 -19.30 -66.13 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -19.30 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: