Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCYGSMSCNPVEQRGEELKYMEKLIELYAQWAGE-------KPSTIEKLEGAGSNRVYYRFGNKS--------GQSVIAVIGTSR--DENHAFIYLTEHFTDKQLPVPQILAVSDDELRYLQTDLGHTSLFDMLKSARDAGGRYTQKEKQLLTKTLQALSEMQVRGAVGLDFQQCYPQPEFNVESVLFDLNYFKYC-FLKATG-IDFH---ELMLEANFRMFAKDLT-AEPAECFLYRDFQARNVMLDND------GNPYFIDYQGGRKGPYYYDLASFLWQASAK----------------YPFKLRRDLVSAYYESLKQYTE-------VPSSRHFVERLNLFVLFRTLQVLGAYGFRGYFERKKHFIQSIPAAMQNLRELIKLGEHVFPYPYMMSVLKEMTELPQFANKEERKVKRTDGFKITDNNIFKANPVDGPATFSKYDDKGELIVRVNSFSYHKGIPEDPTGNGGGYVFDCRSTHNPGRYEPYKNLTGLDEPVIRFLEDDGEILVFLESVYQLADRHVARYIERGFTSLIFSFGCTGGQHRSVYCAQHLAEHIHDKFGIEVRINHREQKIEQTLEAK 5FUT Chain:A ((7-340)) ------------------RTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQ-CSLPDTTATLGDEPRKVLLRLYG--GAEAMVLESVMFAILAERSLG-PKLYGIFPQ--GRLEQFIPSRRLDTEE-----------LSLPDISAEIAEKMATFHGMKMPFNKE----PKWLFGT-MEKY-LKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 954 5462 5.72 21.09 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 5.72 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.181

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: